ht22 mouse hippocampal neuronal cell line  (Procell Inc)

Journal: ACS Omega

Article Title: Research on the Molecular Mechanism of Ginsenoside Ro in Neuronal Damage Following Subarachnoid Hemorrhage

doi: 10.1021/acsomega.5c13079

Figure Lengend Snippet: Effect of Ginsenoside Ro on the mRNA expressions of Bcl-2, EGFR, MMP9, and SRC in HT22 cells (x̅ ± s, n = 3). The mRNA expression levels of Bcl-2, EGFR, MMP9, and SRC between controls and Ro treatment groups were detected by real-time quantitative polymerase chain reaction (A–D). Effect of Ginsenoside Ro on the protein expressions of Bcl-2, EGFR (E–G). Results are shown as x̅ ± s, n = 3, * p < 0.05.

Article Snippet: The mouse hippocampal neuron cell line HT22 ( Mus musculus hippocampal neuron cell line HT22) was purchased from Wuhan Procell Life Technology Co., Ltd. Ginsenoside Ro (CAS No. B21068 , purity ≥98%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: ACS Omega

Article Title: Research on the Molecular Mechanism of Ginsenoside Ro in Neuronal Damage Following Subarachnoid Hemorrhage

doi: 10.1021/acsomega.5c13079

Figure Lengend Snippet: Protective effect of Ginsenoside Ro on hemoglobin-induced HT22 cell damage (x̅ ± s, n = 3). Cell viability was detected by CCK-8 (A); The protein expression of Bcl-2 was detected by Western blot (B). SAH vs Control group,* p < 0.05; SAH vs Ro (100 μM)+SAH, # p < 0.05.

Article Snippet: The mouse hippocampal neuron cell line HT22 ( Mus musculus hippocampal neuron cell line HT22) was purchased from Wuhan Procell Life Technology Co., Ltd. Ginsenoside Ro (CAS No. B21068 , purity ≥98%) was obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China).

Techniques: CCK-8 Assay, Expressing, Western Blot, Control